if one has multiple species , e.g. bound and free, with each a
different relative brightness and anisotropy/polarisation, then the use
( and interpretation) of anisotropy is straight forward; just a sum
weighted by molecular fraction and the relative brightness.
For polarisation is far more complicated.
See e.g. Lakowicz - Principles of fluorescence spectroscopy.
However, as long as one treats & interprets the data correctly there is
no principle reason to forbid the use of polarisation.
Personally, I prefer anisotropy.
Ruud
On 21/7/17 17:57, Keller, Jacob wrote:
*>*Ideally you should convert polarization to anisotropy. Simple
enough – but some referees can get picky…
What is the argument for anisotropy being better?
JPK
--
Ruud Hovius
EPFL SB ISIC LIP
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http://lip.epfl.ch
