Hello everybody, I was looking for suggestion on the best way to identify and refine carbohydrates in a protein. This is the scenario:
- I have 10 molecules in the biological assembly - I used sf9 expression system, so I have random glycolisation and types of glycolisations. I have some hint about possible glycolisation sites - I believe I have only 1 glysolisation per monomer - I have 2 molecule per AU. Space group C2 I would like (even if I have to write down a script for it) to track this glycolisation, identify the most probable ones, determine the restrictions for each and refine them. I could do it by hand on every refinement cycle (or a couple of cycles), but for many datasets it would take ages. What protocol would you suggest? I really appreciate any help in this subject. Regards, Gustavo
