There is a REFMAC plot of Rfactor v resolution, and of <Fobs> v <Fcalc> v resolution. These can show if there is some resolution dependent spike - e.g. one due to ice rings or saturated low resin data. Look at those I suggest Eleanor
On 17 August 2017 at 18:38, Kay Diederichs <kay.diederi...@uni-konstanz.de> wrote: > Dear Rohit, > > in your refinement table I find that the R-factors are elevated in the ice > ring ranges (around 2.22 and 1.93 A resolution). > And your data collections statistics seem to confirm this - CC1/2 is very > high at high resolution which is often due to ice rings. > > Could be a classical case of "data collection problem becomes visible at > refinement stage". > > best, > > Kay > > On Tue, 15 Aug 2017 22:09:44 +0530, rohit kumar <rohit...@gmail.com> > wrote: > > >Dear All, > > > > > > > >I am refining a data in refmac5 and the data resolution is 1.8 A. Data is > >looking fine with the data statics see below for data statics > > > > > > > >[image: Inline image 1] > > > > > > > >Right now the R/Rfree is 22/26 not good for such resolution. I have tried > >most of the options in refmec5 but still I am not able to lower these > >R/Rfree values. > > > >During refinement with phenix what I found that at resolution 1.93 to 1.86 > >R/Rfree is quite high see below > > > > > >[image: Inline image 2] > > > > > > > > > >Is this the possible reason for high R/Rfree value?. If it is please let > >me know how some can remove these frames during refinement. or > > > > > >Please tell me other strategies to lower down the R/Rfree values. > > > > > >Thank you in advance > > > > > >With Regards > > > >Dr. Rohit Kumar Singh > > >
