Hello,
I am refining a few datasets of my protein of interest (ERAP1) bound to various inhibitors, and I repeatedly observe a positive density peak near an asparagine. I first thought this might be a glycan, but this site does not fit the canonical N-linked glycosylation motif (this site sequence is NKL). Previous crystal structures of this protein do not identify any ligand at this position. https://www.dropbox.com/sh/2wdax5uqlb1f4m3/AABQlGK2_mgibtqnm61E-7o0a?dl=0 [https://cfl.dropboxstatic.com/static/images/icons128/folder_dropbox.png]<https://www.dropbox.com/sh/2wdax5uqlb1f4m3/AABQlGK2_mgibtqnm61E-7o0a?dl=0> Asn833 positive density<https://www.dropbox.com/sh/2wdax5uqlb1f4m3/AABQlGK2_mgibtqnm61E-7o0a?dl=0> www.dropbox.com Shared with Dropbox I would appreciate any suggestions of what this density might be. Thank you for your help. Best, Zach Maben Stern Lab Pathology Department University of Massachusetts Medical School <https://www.dropbox.com/sh/2wdax5uqlb1f4m3/AABQlGK2_mgibtqnm61E-7o0a?dl=0>
