Hi Betty,
Could you post a few more screen shots of different orientations of the
electron density?

If you see waters that are 1.6, 1.9 and 2.2 A away from the metal ion, I
would try
Mg2+ ion (trace amount of metal ions could be present in salt
solutions/buffers as an impurity)
and refine it (although 1.6Ă… is too close). In general, Mg2+ ions would
show an octahedral
geometry.





On Tue, Aug 22, 2017 at 3:35 AM, <[email protected]> wrote:

> Dear Betty,
>
>
>
> You have very high resolution, which helps you to identify your ligand,
> but the ligand may be disordered…
>
> What I would do is to place some dummy atoms (e.g. waters) and refine and
> look if the molecule gets clearer. By scrolling the density in coot, you
> can identify the positions with the highest electron density were you
> should put your dummy atoms.
>
>
>
> Looking at your pictures, as far as is possible without the ability to
> rotate them, I would try to fit deoxyribose-phosphate, or even a complete
> nucleotide. Maybe your prep contained some unreacted nucleotides and your
> anomalous peak is phosphorus.
>
>
>
> Good luck!
>
> Herman
>
>
>
> *Von:* CCP4 bulletin board [mailto:[email protected]] *Im Auftrag von
> *Betty Chu
> *Gesendet:* Montag, 21. August 2017 17:19
> *An:* [email protected]
> *Betreff:* [ccp4bb] Unknown positive electron density
>
>
>
> Dear ccp4bb,
>
> I am refining a 1.40 Angstrom data set for a DNA oligonucleotide. While
> the model for the DNA fits very well into the density, there is a patch of
> positive electron density in the solvent space that we are having trouble
> with.
>
> The screenshot can be viewed through this link:
> https://cbsostorage.chem.umd.edu/owncloud/index.php/s/J5cKnOpCC4vb1VC
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__cbsostorage.chem.umd.edu_owncloud_index.php_s_J5cKnOpCC4vb1VC&d=DQMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=oUauCRPJVt0_cKdOakQvtJoEkS4gY9JlJmXfkd2GnlI&s=0XTzg6Yyi4dgz7G80vogTkpLLMZoM8fj53buMeenlJM&e=>
>
> In the screenshot, the yellow color is the anomalous map and a barium ion
> is fitted into density near the positive green electron density.
>
>
> The oligonucleotide was purchased from IDT. The crystallization condition
> is 15% MPD, 120 mM BaCl2, and 30 mM NaCaC pH 6.4. I have tried modelling
> Ba2+ with coordinated waters, MPD, and sodium cacodylate into the electron
> density, but none of those fit well.
>
> Any suggestions regarding the identity of this electron density is much
> appreciated. Thank you!
>
>
>
> Sincerely,
>
> Betty Chu
>
> Paukstelis Research Group
>
> Department of Chemistry and Biochemistry
>
> University of Maryland, College Park
>



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Pradeep Pallan

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