Hi, Sebastiano, I 've never heard about issues with purification of carbohydrates or glycoproteins of this type of sorbent. But I would recommend to perform analytical purification to evaluate your product yield. We usually add 150 mM NaCl to avoin non-specific binding.
2017-09-04 17:41 GMT+03:00 Sebastiano Pasqualato < [email protected]>: > > Hi all, > a group in the department here has a sample with high amount of > maltodextrins (~40 mg/ml) that they would like to load on a superdex column. > Anyone has advices on whether this is doable / not recommendable / > absolutely to avoid? > So far, I have just found that people are separating dextrans on superdex > columns, but their elution buffer is supplemented with 5% ethanol. Is that > added to avoid specific binding of the sugars to the column matrix? Is that > necessary or can it be excluded? > Thanks a lot in advance for any hint, > ciao, > Sebastiano > > > -- > *Sebastiano Pasqualato, PhD* > Crystallography Unit > Department of Experimental Oncology > European Institute of Oncology > IFOM-IEO Campus > via Adamello, 16 > 20139 - Milano > Italy > > tel +39 02 9437 5167 > fax +39 02 9437 5990 > web http://is.gd/IEOXtalUnit > > -- Eugene Osipov Junior Research Scientist Laboratory of Enzyme Engineering A.N. Bach Institute of Biochemistry Russian Academy of Sciences Leninsky pr. 33, 119071 Moscow, Russia e-mail: [email protected]
