Dear all,

My apology for the confusion!! I did mean the map radius is "truncated" at
5A. Poor choice of words :(

Any thoughts on what this density could be are still welcome.

Best regards,
Abhishek

On Fri, Nov 3, 2017 at 9:21 PM, Joern Krausze <[email protected]>
wrote:

> Dear all,
>
> I think Abhishek means that the picture shows 5 A map radius.
>
> Best
> Joern
>
> On 3. Nov 2017, at 18:42, Pavel Afonine <[email protected]> wrote:
>
> If by "it was truncated at 5A for clarity" you really mean you truncated
> all low-resolution data from 5A and lower then I am not surprised you see
> funny densities all over or don't see density where it is expected. Why?
> Consult a textbook for the answer.
>
> All the best,
> Pavel
>
> On Fri, Nov 3, 2017 at 3:10 AM, Abhishek Anan <[email protected]>
> wrote:
>
>> Dear Prof Schreuder
>>
>> Here are another couple of perspectives from coot. The density is too far
>> and isolated from the peptide chain to be an alternate conformation or
>> conformational change. The density of the peptide chain does not look good
>> because it was truncated at 5A for clarity.
>>
>> Best regards
>> Abhishek
>>
>> On Fri, Nov 3, 2017 at 9:33 AM, <[email protected]> wrote:
>>
>>> Dear Abhishek,
>>>
>>>
>>>
>>> To me, it looks like an alternative conformation of the peptide chain or
>>> maybe even a conformational change with respect to the starting model. The
>>> peptide chain does not look too well defined, despite high resolution
>>> electron density.
>>>
>>>
>>>
>>> Best,
>>>
>>> Herman
>>>
>>>
>>>
>>> *Von:* CCP4 bulletin board [mailto:[email protected]] *Im Auftrag
>>> von *Abhishek Anan
>>> *Gesendet:* Freitag, 3. November 2017 09:26
>>> *An:* [email protected]
>>> *Betreff:* [EXTERNAL] [ccp4bb] another unknown density problem
>>>
>>>
>>>
>>> Hi all,
>>>
>>> I have an "unknown" density in the map. I have tried to fit it to PEG
>>> but it doesn't fit very well. I was wondering if there are other
>>> PEG-related or other molecules I could try.
>>>
>>> The crystal grew in TRIS-HCl and PEG MME 2K.
>>>
>>> Thank you
>>>
>>> Abhishek
>>>
>>
>>
>
>

Reply via email to