Hi I am calculating the Electrostatic Potential of my protein. But there were few flexible region with high B-factor and I deleted that part of the protein and then recalculated it. But there I can see a big change.As I have a structure in the presence and the absence of the peptide and the these flexible regions have a better map in the structure without peptide and with peptide I have to delete them. I have a question, should I model these missing regions
or I should ignor them best Jiri