Hi Maria,

What is the refmac version? It should be on the top of the log file.

Regards
Garib


On 29 Jan 2018, at 17:03, Maria Håkansson <[email protected]> wrote:

> Hi,
> 
> I have a question regarding the automatic restraints in Refmac5 and 
> CCP4Interface 7.0.050.
> 
> For a few years I have relied on automatic restraints settings in the CCP4I 
> interface which
> usually resulted in r.m.s. bond deviations of about 0.02 Å and good 
> convergence of refinement (lower
> R-factors). The last few months this have not been the
> case. In structure after structure in different projects we get consistently 
> r.m.s. deviations of 0.03 Å and more
> and lacking or worse convergence. This is not a huge problem since you can 
> get around it by using user 
> set weighting terms. Still it is annoying.
> 
> Anyone experiencing the same?
> 
> Best regards,
> Maria
>  
> 
> Maria Håkansson, PhD, Crystallization Facility Manager
> Principal Scientist
> 
> SARomics Biostructures AB
> Medicon Village
> SE-223 81 Lund, Sweden
> 
> Mobile: +46 (0)76 8585706
> Web: www.saromics.com
> 
> 
> 
> 

Dr Garib N Murshudov
MRC-LMB
Francis Crick Avenue
Cambridge 
CB2 0QH UK
Web http://www.mrc-lmb.cam.ac.uk, 
http://www2.mrc-lmb.cam.ac.uk/groups/murshudov/



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