Just to make sure: The provided definitions are narrowly specific to *enzymes* - it was not specified what protein evoked the critique.
In other cases of apo-use, example apolipoproteins, they are neither enzymes not is the lipid particle what one would commonly associate with a ligand or cofactor. I think it depends to a degree on context and history of the field… Best, BR From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Johannes Cramer Sent: Wednesday, January 31, 2018 6:34 AM To: [email protected] Subject: [ccp4bb] definition of "apo" and alternatives? Dear collegues, a while ago, there was a discussion in the board on the term apo-structure as a way to descibe a native, free, or unbound protein (no ligands). I think the conclusion was that an apo-form is a halo enzyme lacking a cofactor and should not be used as a substitute for "unbound". We were recently asked by a reviewer to change "unbound" to "apo" in a text. We are weighing our options at the moment. Just comply and change it or "teach" the reviewer something... Can anyone share experiences with similar situations? Can anyone point out a publication on the term? Cheers, Johannes
