"Applications of Small Angle Scattering to Structural Biology: An Introduction." On behalf of the organizing committee, we are pleased to announce a workshop on "Applications of Small Angle Scattering to Structural Biology: An Introduction." This workshop will take place on Friday, July 20th, 2018 at this year's American Crystallographic Association (ACA) Meeting in Toronto, Canada. The meeting itself will run from July 20-24. Over the past two decades, SAS has become a mainstay technique for the study of structure and composition in solution for structural biologists around the world. In contrast to the more intensive SAS courses in Europe that extend across several days, this single-day workshop will be comprised of carefully constructed lectures and tutorials to serve as an introduction to investigators new to the technique. The workshop format will include lectures and a selection of hands-on practical exercises. Throughout the workshop the emphasis will be on practical application: knowing how to judge data quality, how to troubleshoot during data collection, and the expectations for a successful experiment and acceptable publication. Students will also learn about aspects of home laboratory data collection and will be introduced to experiments at national user facilities (synchrotrons and research reactors). Students will be expected to bring laptops with the appropriate pre-installed software. Extra laptops will be provided in case of unexpected hardware or software issues on-site, and network connectivity will be provided as part of the course, as some exercises and tutorials will reply on external computer-server resources. Preloaded portable disks and memory sticks will be provided to help reduce the need for large downloads over conference bandwidth. We thank Anton-Paar, Rigaku Corporation, SAXSLAB, and Wyatt Technology Corporation for their kind support of this event. Tentative Program Schedule Time Topic 8:00 AM Registration and Software Installation Help; Speaker Introduction 8:30 AM BioSAS: Overview Why is SAS helpful? 9:00 AM The Scattering Profile Guinier analysis, concentration, monodispersity, etc. 9:30 AM Tutorial 1: Basic Data Analysis I with RAW Is this good data? Radiation Damage? 10:00 AM Coffee Break 10:15 AM Data Analysis: Model-independent Features Molecular Weight Determination, Invariant Plots and Flexibility, The P(r) function 11:00 AM Tutorial 2: Basic Data Analysis II with RAW Calculate a P(r) function, determine Molecular Weight, Dmax, Rg, Volume, etc. 12:00 PM Working Lunch - Laboratory Source SAXS Discussion with Scientists, Experimental Advice 1:00 PM SEC-SAXS Application and Practical Considerations 1:30 PM Sample Preparation Sample purification and preparation, aggregation, buffer selection, etc 1:45 PM Complementary Biophysical Methods for Sample Assessment The importance of supporting biophysical methods including static and dynamic light scattering and analytical ultracentrifugation 2:00 PM Data Analysis: Model-dependent Analysis of SAS Data Calculated scattering profiles from atomic models, Shape reconstruction, Atomistic modeling 2:30 PM Tutorial 3: Three-dimensional modeling from SAS data 3:15 PM Coffee Break 3:30 PM Guest Research Lecture - Thomas Grant, Research Asst. Professor, University of Buffalo/Hauptman-Woodward Medical Research Institute 4:30 PM SANS and Contrast Variation 5:00 PM Publishing SAS data 5:30 PM Conclusion Pre-registration is mandatory. The fee to attend is: * Student (undergrad, grad or postdoctoral) - $185 (CAN) * Corporate - $355 (CAN) * Non-corporate or non-student - $185 (CAN) Lunch will be included. Contact: Kushol Gupta, Perelman School of Medicine, University of Pennsylvania. <mailto:[email protected]> [email protected] Confirmed Instructors: Angela Criswell, Rigaku Corporation Srinivas Chakravarthy, BioCAT Group, Advanced Photon Source Richard Gillilan, Macromolecular Diffraction Facility, Cornell High Energy Synchrotron Source Thomas Grant, University of Buffalo/Hauptman-Woodward Medical Research Institute Jesse Hopkins, BioCAT Group, Advanced Photon Source Maxim Petoukhov, BIOSAXS Group, EMBL Hamburg More information is available at: <https://upenn.us16.list-manage.com/track/click?u=6dbb03108468b01ea4f9d2ad2&; id=e2acf7d04e&e=ca6eaea91e> http://www.amercrystalassn.org/2018-workshops <https://upenn.us16.list-manage.com/track/click?u=6dbb03108468b01ea4f9d2ad2&; id=1a190253ae&e=ca6eaea91e> http://www.amercrystalassn.org/2018-meeting-homepage
