Dear crystallographers, Lately when refining a structure (at 2.8A) with Refmac5 I've found that nearly all Arg residues get distorted at one angle: NE-CZ-NH1(2). Starting model has 120*, final model 123*(117*), which validation server considers a major issue. May any of you recognize why is this happening? Don't remember seeing anything like this before. Current CCP4 version 7.0.050; Refmac5 version 5.8.0189.
Last structure deposited in December'17 did not have those issues. CCP4 version then was 7.0.047; Refmac5 version was the same. Thank you for your time. Regards, Vaheh Oganesyan MedImmune, ADPE www.medimmune.com To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation.