Hi all, I'm curious about which kind of chromatography set-up people are using for running size-exclusion chromatography as a final purification step prior to vitrification and cryo-EM.
Especially for scarce samples/small volumes, you'd want to have a system with minimal dead volumes and the ability to fractionate small volumes (say <40-50 µL) accurately, so as to minimize sample dilution. Does anyone do this on an HPLC system as opposed to classical FPLCs? Thanks! Bastian