Hi all,

I'm curious about which kind of chromatography set-up people are using for 
running size-exclusion chromatography as a final purification step prior to 
vitrification and cryo-EM. 

Especially for scarce samples/small volumes, you'd want to have a system with 
minimal dead volumes and the ability to fractionate small volumes (say <40-50 
┬ÁL) accurately, so as to minimize sample dilution.

Does anyone do this on an HPLC system as opposed to classical FPLCs?



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