>
> hi
> you can find details in this manuscript on how to label recombinant proteins
> produced by P. pastoris
> Crystal structure of the effector AvrLm4-7 of Leptosphaeria maculans reveals
> insights into its translocation into plant cells and recognition by
> resistance proteins. <https://www.ncbi.nlm.nih.gov/pubmed/26082394>
> Blondeau K, Blaise F, Graille M, Kale SD, Linglin J, Ollivier B, Labarde A,
> Lazar N, Daverdin G, Balesdent MH, Choi DH, Tyler BM, Rouxel T, van Tilbeurgh
> H, Fudal I.
>
> Plant J. 2015 Aug;83(4):610-24. doi: 10.1111/tpj.12913. Epub 2015 Jul 14.
>
> it is not easy to obtain a homogenous labeled sample, but if there are enough
> methionines in you protein it might work
> all the best
>
>
> Herman van Tilbeurgh
> Professor structural biology
> Directeur Adjoint Ecole Doctorale Innovation Thérapeutique: du fondamental à
> l'appliqué
>
> Institut de Biologie Intégrative de la Cellule - I2BC
> UMR 9198 CNRS- Université Paris Sud
> Team: Fonction et Architecture des Assemblages MacroMoléculaires
> http://www.i2bc.paris-saclay.fr/spip.php?article256
> <http://www.i2bc.paris-saclay.fr/spip.php?article256>
>
> Batiment 430
> 91405 Orsay
> France
>
> Tel: 33 1 69 82 61 16
> [email protected] <mailto:[email protected]>
>
>
>
>
>
>> Le 29 mars 2018 à 00:32, Anirban Banerjee <[email protected]
>> <mailto:[email protected]>> a écrit :
>>
>> Dear all,
>>
>> There is not much by way of published literature on this. Are there
>> unpublished methods that people have used and have found to work reliably ?
>>
>> Any help will be very much appreciated.
>>
>> Warm regards,
>>
>> Anirban
>
