Dear JiYG, Just to mention also that it was impressed on me in a similar situation, either in my reading a ccp4bb thread or Randy directly, that the new version of Phaser (early 2017 onwards) was improved for such situations. Since you will no doubt keep your Phaser up to date anyway you should be all set to proceed ok. Best wishes, John
Emeritus Professor John R Helliwell DSc > On 8 May 2018, at 15:50, herman.schreu...@sanofi.com wrote: > > Dear JiYG, > > Unless the tNCS has caused processing problems, Phaser should automatically > deal with tNCS and I would recommend to just give it a try. If it fails, you > could try more sophisticated approaches. > > Best, > Herman > > Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von ??? > Gesendet: Dienstag, 8. Mai 2018 16:41 > An: CCP4BB@JISCMAIL.AC.UK > Betreff: [EXTERNAL] [ccp4bb] tNCS problem > > Dear all, Recently, I collected a data set of a crystal of a big protein, > which contains 1000 amino acids. I processed the data to P2. But the data set > has tNCS problem. I want to do molecular replacement. Is there anyone know > how to deal with this problem? Thanks, JiYG > >
