Hi, While doing a precise magnification calibration, as described in the article in the link below, I get two different values one at 1.049 and the other at 1.045 for two different datasets. I have to eventually compare the pdbs from these two datasets. My question is: should I process the final maps for the two datasets at the above mentioned two different values?
Thanks, On Sun, Feb 25, 2018 at 10:22 AM, Bruno KLAHOLZ <[email protected]> wrote: > > > Dear Reza, > > > > you can do this kind of precise magnification calibration by fine-scaling > the cryo-EM map with respect to your atomic model using for example > Chimera. Try varying the scales by increments of 0.01 of the pixel size and > check for the best cross-correlation between the cryo-EM map and a map > calculated from the atomic model. > > Btw, we noticed that the quality of this fine calibration is also visible > in the geometry quality of the atomic model under refinement (Ramachandran > outliers etc.), e.g. when using Phenix, Buster etc. > > > > See for example methods section in https://www.ncbi.nlm.nih.gov/ > pubmed/29143818 > > > > Best regards, > > > > Bruno > > > > > > *From:* CCP4 bulletin board [mailto:[email protected]] *On Behalf Of *Reza > Khayat > *Sent:* 24 February 2018 21:38 > *To:* [email protected] > *Subject:* [ccp4bb] EM voxel size correction > > > > Hi, > > > > Has anyone established a robust method to calibrate the pixel/voxel size > of a cryo-EM image reconstructions using a known PDB? Thanks. > > > > Best wishes, > Reza > > > > Reza Khayat, PhD > > Assistant Professor > > City College of New York > > Department of Chemistry > > New York, NY 10031 > -- SS ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
