It does happen that “beautiful” crystals have no diffraction. Here is an example : https://www.sciencedirect.com/science/article/pii/0022283691906078
In my experience, crystals are fairly stable. But not always: crystals of human endothelial nitric oxide synthase or inducible nitric oxide synthase catalytic domain lasted two weeks at most before losing their diffraction completely. The method used to freeze the crystal can be a factor. It is worth checking at room temperature. But your crystals are not necessarily a complete loss: they can be used as micro-seeds added to a broad screen, and help discover new crystal forms. Thierry From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Careina Edgooms Sent: Tuesday, August 14, 2018 5:59 AM To: [email protected] Subject: [ccp4bb] crystals that dont diffract :( :( EXTERNAL EMAIL – Use caution with any links or file attachments. I got the most beautiful crystals I have ever seen and they don't diffract at all. Not poor diffraction, NO diffraction. Anyone know why this could be and how I can go about fixing it? I had three beautiful crystals and not one diffracted. I did leave them in the drop for about 3 weeks before harvesting and in liquid nitrogen for about a month before diffracting. Could that be a factor? If I regrew more beautiful crystals and diffracted straight away could that help? Careina ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
