Dear Xavier,

in addition to Rob and Israel's excellent suggestions you may also want to
have a look at Garib's account in Chapter 11 here:

https://www.sciencedirect.com/bookseries/methods-in-enzymology/vol/579

Best wishes,
Arjen

On Friday, 31 August 2018, F.Xavier Gomis-Rüth <[email protected]> wrote:

> Dear all,
>
> is there some kind of general trend if not consensus as how to refine
> cryo-EM structures? What do people do for maps in the 3-4Å range,
>
> pure RSR against the untouched map and that's it? Reciprocal space
> refinement against the backtransformed structure factors from the map
>
> but without touching the map?
>
> I've even heard that some people "dare" to calculate 2mFo-DFc-maps....
>
> Thanks a lot for your feedback.
>
> Best,
>
> Xavier
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