Hi Tobias, I don't know of any specific claims made about this but you'd better check the data yourself if you find an example. A nice paper by Bill Hunter and his team (http://scripts.iucr.org/cgi-bin/paper?S1744309111005835) showed a case where Co2+ was an essential ion for crystallisation , but it was overlooked by the original depositors of the model. Not being in a model does not show that it's really not there.
Cheers, Robbie > -----Original Message----- > From: CCP4 bulletin board <[email protected]> On Behalf Of Tobias > Beck > Sent: Tuesday, September 11, 2018 13:42 > To: [email protected] > Subject: Re: [ccp4bb] Literature on crystallization screen ingredients not > showing up in crystal structure > > Thanks for the replies I have received so far (on and off list). > > I would like to emphasize an important aspect: What about crystallization > trials where the ingredients do not show up, but cannot be omitted for the > crystallization, e.g. buffers to control pH, as mentioned in one response - I > am also thinking of specific ions, that are required, but are disordered - any > general reference for this? > > > > Can't you just point out that crystallization is a purification method > Yes, that would be one way to put it. > > Thanks, Tobias. > > > > On Tue, Sep 11, 2018 at 12:38 PM Patrick Shaw Stewart > <[email protected] <mailto:[email protected]> > wrote: > > > > Can't you just point out that crystallization is a purification method? > E.g. look at the number of proteins in the Sigma catalogue that have been > purified by crystallization. Clearly crystallization often excludes other > solutes > in the mix. > > Best wishes, Patrick > > > On Tue, 11 Sep 2018 at 08:41, Tobias Beck <[email protected] > <mailto:[email protected]> > wrote: > > > Dear all, > > I am looking for some general references regarding the fact > that for a crystallization condition not all ingredients of the > crystallization > cocktail will show up in the crystal structure. Ions could be disordered, > buffer components located in the solvent regions, etc. > > > I think this is rather common knowledge, but maybe there is, > especially regarding protein crystallization, a more general reference (apart > from text books on solubility) for this and I am just not using the right > search > terms... > > > Thank you! > > Best, Tobias. > > -- > > ___________________________________________ > > Dr. Tobias Beck > - independent group leader - > RWTH Aachen University > Institute of Inorganic Chemistry > Landoltweg 1, office: 304N > 52056 Aachen, Germany > phone: +49-241-80-90057 > fax: +49-241-80-99003 > web: http://www.ac.rwth-aachen.de/extern/beck/ > ___________________________________________ > > > > ________________________________ > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi- > bin/webadmin?SUBED1=CCP4BB&A=1 > > > > -- > > [email protected] <mailto:[email protected]> Douglas > Instruments Ltd. > Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK > Directors: Peter Baldock, Patrick Shaw Stewart > > http://www.douglas.co.uk > Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 > Regd. England 2177994, VAT Reg. GB 480 7371 36 > > > <http://t.sidekickopen13.com/e1t/o/5/f18dQhb0S7kC8dDMPbW2n0x6l2B9g > XrN7sKj6v5KRN6W56jV385v7rnbW4WDX_q2HNFv8f197v5Y04?si=700000000 > 1398361&pi=d783306d-9ddd-4cae-fcf9-ebed897fee90&ti=null> > > > > -- > > ___________________________________________ > > Dr. Tobias Beck > - independent group leader - > RWTH Aachen University > Institute of Inorganic Chemistry > Landoltweg 1, office: 304N > 52056 Aachen, Germany > phone: +49-241-80-90057 > fax: +49-241-80-99003 > web: http://www.ac.rwth-aachen.de/extern/beck/ > ___________________________________________ > > > > ________________________________ > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
