Hi Almudena, I wonder to which extent I can trust the positive blobs or polder maps that > I am generating... >
the answer to this question is given in corresponding paper that describes Polder map: http://journals.iucr.org/d/issues/2017/02/00/ba5254/ba5254.pdf Based on the analysis described in this paper the program states the verdict in plain English (as opposed to a bunch of numbers). For a lazy option you can watch the video tutorial https://www.youtube.com/watch?v=TcTuMJayh5c&feature=youtu.be For even lazier option you can fast-forward to minute 4 of the video where Dorothee shows what to watch for. > Anybody that can make other suggestions concerning how to validate the > ligand binding site and/or generate other maps that could be more helpful? > If you have another data set collected from the crystal without the ligand and crystals are reasonably isomorphous, you can calculate Fobs-Fobs map. Good luck, Pavel ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
