Dear colleagues: For those of you interested in cryo-EM, Matthijn Vos and I would like to announce that we have just made a large set of new training videos public covering the operation of Arctica and Titan Krios microscopes. The videos are part of an ongoing expansion of the original “Getting Started in Cryo-EM” video series some of you may already be familiar with. The new videos we are releasing today begin with a comprehensive introduction to every control panel and button on the user interface, then cover gun alignment, the condenser system, how to obtain parallel illumination on both the Arctica and Krios, consequences of not having a parallel beam, different ways to focus, projection and diffraction issues, history and differences between all kinds of detectors, issues surrounding energy filtration, how to quickly assess the performance of your microscope, what to look for when screening samples, and how to set up single particle data collection with EPU. The demonstration videos are full screen captures of the monitors plus views of the hand panels, so viewers can see all the actions taken by the operator in sequence and their consequences, while they are being explained in real time. In addition, the demonstration videos are complemented with many new lecture-style theory videos presented through drawings and images on a tablet, as in the original Getting Started in Cryo-EM lectures.
Together the whole package is now ~70 hours of video, which we hope you will find helpful in your training. The videos are all released free of charge with the Creative Commons license CC BY 4.0, so you can download, copy, redistribute, remix, and otherwise use as you see fit as long as you credit the original source. Their production was funded in part by the NIH Transformative High Resolution Cryo-Electron Microscopy Program (grant R25 EY-029128 to Grant Jensen) and in part by ThermoFisher Scientific. Because there are now so many videos, we have arranged them in different modules on a customized website that keeps track of your progress and that will allow us to add self-test questions in the future. Please find the videos at https://em-learning.com As for many other NIH- and/or company-funded resources, the website requires a simple registration to help us gather statistical information about what kinds of individuals are using the materials. Please also send us (either Matthijn or me, both copied above) email directly about any suggestions, comments or corrections you have – this will help us improve the videos and will give us the feedback we need for our reports to the NIH. Best regards, Matthijn Vos and Grant Jensen -------------------------------------------------------------------------- Grant Jensen Professor of Biology and Biophysics and Investigator, HHMI California Institute of Technology, Mail code 114-96 1200 E. California Blvd. Pasadena, CA 91125 626-395-8827 (phone) 626-395-5730 (fax) JensenATcaltech.edu http://www.jensenlab.caltech.edu office location: 359 Broad (northwest corner of campus) Administrative Assistant Karin Mallard 626-395-8893 (phone) kmallardATcaltech.edu -------------------------------------------------------------------------- ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
