Unit cell dimensions don’t really matter here. If it’s a centred lattice you 
won’t be able to index it correctly in a primitive system even though C222 and 
P222 both have a<>b<>c and alpha=beta=gamma=90.

Ditlev

---

Ditlev E. Brodersen
Lektor, Associate Professor
Department of Molecular Biology and Genetics, Aarhus University
Gustav Wieds Vej 10c, DK-8000 Aarhus C, Denmark

Phone:  +45 21669001
Lab home page: www.bioxray.au.dk/~deb<http://www.bioxray.au.dk/~deb>
Educational IT portal: curriculearn.dk<http://curriculearn.dk>

Sent from my iPhone

Den 29. nov. 2018 kl. 18.25 skrev Damodharan Lakshminarasimhan 
<[email protected]<mailto:[email protected]>>:

Hi Vijyay,

Did you tried with P212121 system?. RCSB has one structure with similar 
unitcell and the corresponding pdb code is 3BMA. If you have not tried then I 
hope it will solve the problem.

Thanks
damo

On Thu, Nov 29, 2018 at 11:44 AM Vijay Jayaraman 
<[email protected]<mailto:[email protected]>> wrote:
Hi all,
The data that we collected recently is in space group C 2 2 21 and appears to 
be twinned. I could not find a twin law which I can use to detwin the data. The 
R and R free doesn't drop below 0.4 even after multiple rounds of manual and 
automated refinement.  I have also attached the self-rotation function output 
as a pdf file here. Kindly suggest a solution. Useful details are here:

Space group C 2 2 21
Unit cell:    92.24  148.78  206.60      90.00   90.00   90.00
Maximum resolution: 2.6 Angstrom

This is the warning message from the AIMLESS log file:

"WARNING: the L-test suggests that the data may be twinned,
   so the indicated Laue symmetry may be too high
   Rough estimated twin fraction alpha from cumulative N(|L|) plot 0.151 
+/-(0.020)
   Rough estimated twin fraction alpha from     0.141
   Rough estimated twin fraction alpha from     0.128"

Thanks.


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