Dear colleagues, The Nakanishi lab is looking for a self-motivated structural biologist interested in elucidating the molecular mechanisms of diversified RNA silencing by human Argonaute proteins and their binding partners. The position is available immediately and will remain open until filled.
MicroRNAs and other small RNAs are loaded into different Argonaute proteins to form unique effector complexes, regulating many biological processes. Humans have four Argonaute paralogs, each of which is responsible for specialized roles. Our focus is to understand the molecular bases for their unique RNA silencing. To this end, we take a combinatorial approach of molecular biology, biochemical and biophysical methods, and structural biology (crystallography and cryo-EM). Candidates should have a strong background and expertise in X-ray crystallography or single-particle electron microscopy and the biochemical experiments. Applicants with experience in RNA biology will be stronger candidates. New Ph.D. graduates are encouraged to apply. My group has a great environment to pursue goals. We own multiple AKTA FPLC systems and an automatic crystal plate imaging system. We have access to OSU Campus Chemical Instrument Center in the same building that has a crystallization robot (Mosquito) and a laboratory-scale X-ray diffractometer (Rigaku MicroMax-003 with a Pilatus 200K detector). We remotely carry out our crystallographic experiments at The Advanced Photon Source. The Ohio State University has a state-of-the-art cryo-EM facility, CEMAS (URL: https://cemas.osu.edu), that is located within a 5 min driving distance from my lab. There is a Titan Krios with a K3 Bioquantum direct electron detector, imaging phase plates, STEM/TEM tomography, and an image spherical aberration corrector. Interested candidates should email a cover letter describing their research experience, a curriculum vitae, and the names of three references (one should be the current supervisor) to [email protected]. Best, Kotaro Kotaro Nakanishi, Ph.D. Assistant Professor Department of Chemistry & Biochemistry The Ohio State University 742 Riffe Building, 496 W 12th Avenue Columbus, OH 43210-1292 Tel: (614)-688-2188 E-mail: [email protected] Web: http://u.osu.edu/nakanishilab/ Twitter: https://twitter.com/nakanishi_lab ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
