Hello Chandra, Soaking heavy atoms into crystals has a long and successful history (I use it pretty often and it works much of the time). If you need phase information and you have some crystals, I would certainly give it a try. There are many papers and books to give you methods to do this (good old Blundell and Johnson for one, Methods in Enzymology for another). No reason to waste good native crystals if you still have some ;-) Cheers, tom
Sent remotely On 3 Jan 2019, at 6:43 am, Chandramohan Kattamuri <[email protected]<mailto:[email protected]>> wrote: Dear CCP4 members, Can someone suggest to me a methodology to covalently modify (non-cysteine) on component of a multicomponent complex with heavy atoms in order to achieve better phase determination? Thanks in advance Chandra [email protected]<mailto:[email protected]> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
