Hi Erik, Have you tried Achesym?
http://achesym.ibch.poznan.pl Kowiel, M., Jaskolski, M. & Dauter, Z. (2014). ACHESYM: an algorithm and server for standardized placement of macromolecular models in the unit cell. Acta Cryst. D70, doi:10.1107/S1399004714024572. Best Ricardo Padua On Sat, Feb 2, 2019 at 4:06 PM Klontz, Erik <[email protected]> wrote: > Hi all, > > > I'm working on a protein that I believe is tetrameric based on AUC, gel > filtration, and crystallography. However, although my asymmetric unit has 4 > chains, I cannot form the tetramer within the asymmetric unit. Instead, the > asymmetric unit has two half-tetramers ('dimers'), and each full tetramer > is completed by pairing up with another half-tetramer from a symmetry mate. > If I load this structure into PISA, it recognizes that each of the 'dimers' > forms a stable assembly, but cannot seem to assemble the tetramer. However, > if I the generate symmetry mates in pymol to create a new PDB for the > biological tetramer and give this to PISA, it recognizes a stable tetramer. > > > Specifically, chains A and C in the original PDB pair with chains A and C > of the second symmetry mate generated in pymol, while chains B and D in the > original pair with chains B and D of the third symmetry mate. How do I use > this knowledge to generate a 3x4 rotation with translation matrix for PDB > deposition? > > > Thanks, > Erik Klontz > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- Ricardo Padua HHMI Research Associate Kern Lab Dept. of Biochemistry, MS009 Brandeis University Waltham, MA 02454, USA E-mail: [email protected] ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
