Let's say I have a protein crystal from which I collected 30 datasets. If I
plot the unit cell volume per dataset the volume rises.
My question is: Is there a rule of thumb of some sort* to consider the
initial/final datasets isomorphous still?

* Something like if the unit cell volume changes more than 1% then the
crystal is not isomorphous.

My second question is: Meents already said that the unit cell volume
expansion is a consequence of hydrogen gas building up inside the crystal.
But...what if the unit cell volume decreases? Is there an explanation for
that?


Thank you very much.

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