Dear Colleagues, Recently I solved one Ab-Ag complex structure. It was indexed as p212121 or p21212 space group and solved the structure with one Fab and one Ag in the cell unit. The contact interface is not the one I expected (elbow region contacts with Ag). Using symexp I got several symmetry related molecules, showing the interface of antibody CDR with the antigen.
For non-structural biologist it is quite tricky to display PDB file with symmetry related files. To make things simple, I want to solve the structure with the biologically relevant complex in one AU, However, changing space groups (such as P1 or P2) didn't help and I failed in the following steps. Some people really added the symmetry related molecules into the final pdb (merge and rename the chain ID of the mate molecules). So is it acceptable in structure community? Many thanks. Best Regards, Jingxian ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
