I am not sure if you have tried seeding as well. That helped me in growing bigger crystals. Best, Bharti Singal
[http://www3.ntu.edu.sg/exchange/outlook2007/composeandsendmsg/ntulogofit2.gif] Bharti Singal | PhD Student | Division of Structural Biology & Biochemistry | Nanyang Technological University SBS-03s-48, 60 Nanyang Drive, Singapore 637551| Tel: (65) 6316-2926 GMT+8h | Email: [email protected]<mailto:[email protected]> | Web: www.ntu.edu.sg<http://www.ntu.edu.sg/> ________________________________ From: #SINGAL BHARTI# Sent: Friday, March 22, 2019 2:32 PM To: [email protected]; zhangyi19950109 Subject: Re: [ccp4bb] RDRP crystal Hi Zhangyi, You can try using capillary counter diffusion method. Not sure if it can help, but you can give it a try. You can check the following paper. https://www-sciencedirect-com.ezlibproxy1.ntu.edu.sg/science/article/pii/S1047847703000522?via%3Dihub#FIG2 Best, Bharti Singal [http://www3.ntu.edu.sg/exchange/outlook2007/composeandsendmsg/ntulogofit2.gif] Bharti Singal | PhD Student | Division of Structural Biology & Biochemistry | Nanyang Technological University SBS-03s-48, 60 Nanyang Drive, Singapore 637551| Tel: (65) 6316-2926 GMT+8h | Email: [email protected]<mailto:[email protected]> | Web: www.ntu.edu.sg<http://www.ntu.edu.sg/> ________________________________ From: CCP4 bulletin board <[email protected]> on behalf of zhangyi19950109 <[email protected]> Sent: Friday, March 22, 2019 2:21 PM To: [email protected] Subject: [ccp4bb] RDRP crystal Dear all, A single positive stranded RNA virus RDRP monomer grows crystals, precipitants (PEG8000) with low concentration, more nucleuses and poor resolution.Reduce precipitant concentration can reduce the crystal nucleus, but worse resolution, precipitant concentration increased, many crystal nucleus and crystal is very small. Try to optimize the temperature, pH, protein concentration, dehydration, additives, detergent, sit and hanging drop, but were not improved much. Pease provide some advice methods. Thanks, Yi Zhang This is a picture of this crystal [http://mail.163.com/js6/s?func=mbox:getMessageData&sid=*&mid=69:1tbiRQd-6Vl9pcIeZAACsv&part=3] ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
