Try seeding at lower concentration. From: CCP4 bulletin board <[email protected]> On Behalf Of zhangyi19950109 Sent: Friday, March 22, 2019 1:55 AM To: [email protected] Subject: [ccp4bb] image
Dear all, A single plus-stranded RNA virus RDRP monomer grows crystals, precipitants (PEG8000) with low concentration, more nucleuses and poor resolution.Reduce precipitant concentration can reduce the crystal nucleus, but worse resolution, precipitant concentration increased, many crystal nucleus and crystal is very small. Try to optimize the temperature, pH, protein concentration, dehydration, additives, detergent, sit and hanging drop, but were not improved much. Pease provide some advice methods. This is a picture of this crystal [cid:[email protected]] ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://clicktime.symantec.com/3FMTo7fSnEQhb6QpGBMfnqq7Vc?u=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1> To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
