On 02/04/2019 14:28, Sam Tang wrote:
Hello
Hello.
I have been refining a protein-RNA complex with Refmac. The RNA we used was tagged with a Cy label. I
modelled the RNA in Coot using the RCrane add-on and manually built the Cy tag. However after Refmac (also
via Coot) the linkage between Cy and the RNA was always broken.
I guess I must have missed something obvious.
Maybe.
What CCD entry are you using for your Cy tag? What have you done to specify the link between the Cy and the
RNA? What do you mean by "broken"? That the LINK is missing or that the distance between the linked atoms is
too large? What did the refmac log file tell you about the distance between the atoms that you thought
should be linked? Have you made a link using Acedrg in CCP4i2? Or Coot? (Extensions -> CCP4 -> CCP4 -> Make
a Link via Acedrg)?
Regards,
Paul.
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