Hello Nemanja, I used to wash and reuse glass plates for neutron crystallography. Of course glass is sturdier than polystyrene, but I can't think of any protein stain that would resist a treatment with detergent, then a strong base (say 0.1M NaOH) and finally a strong acid (say 0.1M HCl)... Regarding the drop shape problem that Janet mentioned, we applied Sigmacote to the dry clean plates, a siliconizing agent sold by Sigma Aldrich, which turns the surface non-adherent and chemically inert. From the website: *Sigmacote® is a solution of a chlorinated organopolysiloxane in heptane that readily forms a covalent, microscopically thin film on glass. The film repels water, retards the clotting of blood or plasma, and prevents surface adsorption of many basic proteins.* However, I don't know whether polystyrene would resist the heptane solvent, but the applied coat is very thin and should evaporate quickly if let dry in a hood with the fan on. I hope this helps and please let me know if it works!! Best wishes, Javier
On Wed, Apr 10, 2019 at 8:11 PM Newman, Janet (Manufacturing, Parkville) <janet.new...@csiro.au> wrote: > Hi Nemanja, > > > > I have tried doing this, and it has never really worked for me, even with > careful rinsing with MilliQ water after washing, I could never get > well-shaped drops on a recycled plate. They are also a real pain to wash > out, and it’s hard to get the last traces of protein out of the subwells > without scratching the subwells. (I was also doing this with the > polystyrene SD-2 plates from SwissSci) > > > > Janet > > > > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of > *Nemanja > Vuksanovic > *Sent:* Thursday, 11 April 2019 4:42 AM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] Re-using 96-well crystallization plates > > > > Dear All, > > > > I'd like to ask if anyone has experience cleaning old 96 well > crystallization plates? I have a large number of old plates (Swissci) with > mostly INDEX and PEG Ion screens and I thought of re-using them instead of > throwing them away, but I'm not sure if this would be viable. > > > > Best regards, > > > > Nemanja Vuksanovic > > > -- > > Graduate Student > > Department of Chemistry and Biochemistry > > University of Wisconsin-Milwaukee > > > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- Dr. Javier M. González Instituto de Bionanotecnología del NOA (INBIONATEC-CONICET) Universidad Nacional de Santiago del Estero (UNSE) RN9, Km 1125. Villa El Zanjón. (G4206XCP) Santiago del Estero. Argentina Tel: +54-(0385)-4238352 Email <bio...@gmail.com> LinkedIn <https://www.linkedin.com/in/javier-m-gonzalez-inbionatec> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
