Re: question 2, for simplicity, I would call it all one helix and regard the 
3-10 part as a distortion. Just my opinion. 

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  On Tue, 28 May 2019 at 13:48, Paul Emsley<[email protected]> wrote:   
On 28/05/2019 11:51, Stephan Grunwald wrote:
> 
> I [have] recently solved [my] first X-ray structure and I would like to have 
> an opinion on 
> two questions, both dealing with secondary structure assignments.
>
> First of all, the wwPDBs says ”We encourage authors to use the calculated 
> helix (or sheet) records and not 
> provide their own remarks.” (https://www.wwpdb.org/documentation/procedure). 
> It says in the same 
> documentation, that the Promotif software will automatically assign SSE upon 
> deposition (does Promotif use 
> the DSSP algorithm?).

1) Your are encouraged, not mandated. ProMOTIF/dssp is one of a number of ways 
to generate secondary 
structure annotations. You seem to have a good reason to use "your own" 
assignment. Go ahead and do so, 
don't give it a second thought.

I don't have an opinion about question 2.

Paul.

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