It is very difficult to demonstrate rigorously that crystal packing does not favor a particular conformation. If you can show that the same conformation occurs in solution (SAXS, NMR), or that the same conformation is found in a different space group with different packing, then you have a pretty conclusive answer. Obviously this involves a great deal of extra effort.
> On 28 May 2019, at 9:59 AM, Zizi Tian <[email protected]> wrote: > > Hi All, > I has recently solved my X-ray structures of the ZnF domains of protein and > its complex with dsDNA. A structural comparison of holo-protein and the > protein-DNA binary complex reveals that the relative orientation of domains > ZnF3 undergoes a rotation of 50.5 degrees Does anyone know how to show there > are no effects of the crystal packing . > > Please help me in this regard. > > Best regard > > > > > > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> Patrick Loll [email protected] ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
