Hello James, I like your list, but I wonder how much of this can be covered in meaningful depth during a single GRC.
Your item >7) what is the best way to process serial crystallography data? deserves differentiation; there is a huge gap between serial synchrotron crystallography (SSX) with rotation of a few degrees, and serial femtosecond crystallography (SFX) at X-FELs. And stills collected at a synchrotron has aspects of both techniques. best, Kay On Mon, 15 Jul 2019 19:44:03 +0000, Holton, James M <[email protected]> wrote: >Hello folks, > >I have the distinct honor of chairing the next Gordon Research >Conference on Diffraction Methods in Structural Biology (July 26-31 >2020). This meeting will focus on the biggest challenges currently >faced by structural biologists, and I mean actual real-world >challenges. As much as possible, these challenges will take the form of >friendly competitions with defined parameters, data, a scoring system, >and "winners", to be established along with other unpublished results >only at the meeting, as is tradition at GRCs. > >But what are the principle challenges in biological structure >determination today? I of course have my own ideas, but I feel like I'm >forgetting something. Obvious choices are: >1) getting crystals to diffract better >2) building models into low-resolution maps (after failing at #1) >3) telling if a ligand is really there or not >4) the phase problem (dealing with weak signal, twinning and >pseudotranslation) >5) what does "resolution" really mean? >6) why are macromolecular R factors so much higher than small-molecule ones? >7) what is the best way to process serial crystallography data? >8) how should one deal with non-isomorphism in multi-crystal methods? >9) what is the "structure" of something that won't sit still? > >What am I missing? Is industry facing different problems than >academics? Are there specific challenges facing electron-based >techniques? If so, could the combined strength of all the world's >methods developers solve them? I'm interested in hearing the voice of >this community. On or off-list is fine. > >-James Holton >MAD Scientist > > >######################################################################## > >To unsubscribe from the CCP4BB list, click the following link: >https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
