Dear Jessica, There's a dedicated Phenix bulletin board, where it would be better to post Phenix-specific questions.
Nonetheless, you can do this in both the CCP4 and Phenix interfaces to Phaser, so I'll explain how to do this in all the interfaces. First, I'm guessing that the order of the tNCS wasn't unambiguous, that Phaser chose something other than NMOL 3 in the automated run, but now you want to test the alternative of NMOL 3. ccp4i: Under the "User parameters" tab, change "Number of assemblies related by TNCS vector" to 3. The toggle should automatically be checked when you do that. ccp4i2: In the Expert Mode Phaser MR GUI, under the "Keywords" tab, change "Number of molecules or complexes related by translation vector" to 3. Phenix: in the Phaser-MR (full-featured) GUI, "Input and general options" tab, click on "Other settings". In the "Translational NCS" section of the popup, set "Number of molecules or complexes related by translation vector" to 3. And for completeness, if you're writing a command-line script, the command is "TNCS NMOL 3". Best wishes, Randy Read > On 23 Jul 2019, at 16:06, Jessica Besaw <[email protected]> wrote: > > Hello everyone, > > Does anyone know of a good phenix tutorial on how to deal with higher order > translational non-crystallographic symmetry (tNCS) in a structure? > > Ultimately, I need to know how to set TNCS NMOL to 3 in phaser of Phenix. I > am using the phenix GUI, and I am uncertain how to alter this parameter. > > Any help would be greatly appreciated > > Cheers! > > Jessica > > > > > <http://www.avg.com/email-signature?utm_medium=email&utm_source=link&utm_campaign=sig-email&utm_content=webmail&utm_term=oa-4885-a> > Virus-free. www.avg.com > <http://www.avg.com/email-signature?utm_medium=email&utm_source=link&utm_campaign=sig-email&utm_content=webmail&utm_term=oa-4885-a> > <x-msg://228/#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ------ Randy J. Read Department of Haematology, University of Cambridge Cambridge Institute for Medical Research Tel: + 44 1223 336500 The Keith Peters Building Fax: + 44 1223 336827 Hills Road E-mail: [email protected] Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
