Hello everyone >From electron density, It looks like one of the arginines in my protein is post-translationally modified as phospho-arginine. How to make sure it's a real phospho-arginine, not an arginine interacting with phosphate or acetate ion? The resolution of the structure is 2.3 A. Can anybody helps me with step by step procedure to incorporate a phospho-arginine in my structure using COOT and if anything special need to be done during refinement in Phenix. Thanks and regards Sincerely Anirudha Dutta
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