Ah, low resolution experimental phasing. Fun stuff, seems like a lost art these days.
Are the crystals large ? Is it possible to do a (continuous helical data) collection? (Manually or if your beamline permits you to). Granted this is anomalous, is it possible to then merge enough partial datasets together? (Very carefully of course). If not there’s always SIRAS. With this rapid decay, are you using a strategy? (Another lost art these days) Given the difficulty of this project, have you pursued other derivatives (via soaking)? > On Aug 29, 2019, at 11:26 AM, Kimberly Stanek <ksta...@uci.edu> wrote: > > Hi folks, > > We have a protein that we have been trying to solve the structure of for a > while now but so far haven't been able to get any diffraction better than > ~4.5A. I was able to collect a full 360 degrees of data and index, but MR is > failing so we have turned to de novo phasing. > > Recently we prepared crystals of the Semet derivative under the same > condition. While these crystals diffracted to about the same resolution, I > found they were dying after just one or two snaps, even with increased beam > attenuation and decreased exposure time. I am wondering if anyone has > experienced anything like this before and had any suggestions on what to do > about it. > > Thank you, > > > > Kimberly Stanek > Postdoctoral Researcher, Goulding Lab > Co-chair, UCI Postdoctoral Association > University of California, Irvine > Natural Science I, Room 2302 > (949) 824-0014 > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
