No. As pointed out by Pavel, there are no reflections flagged as "free reflections" used to calculate Rfree. Therefore you don't get a real Rfree. No model bias issue etc. If you have a Rfree using ccp4 programs, than you should used that particular original mtz in phenix. It should detect the flagged "free reflections" automatically.
Cheers Christian On Fri, Aug 30, 2019 at 5:24 PM Tung Thanh Dinh <[email protected]> wrote: > I have been using elipsoidal truncated data all long. And at first i > couldn't find a well homologous model (50% homology is the best i can find) > so I use Mr Bump and Buccaneer to create a model to phase and autobuild. > After a lot of refinement I obtained "good" model with good statistics. > Then I used this "good" model to either phase and autobuild again. So this > may be due to the model bias? > > > Kindest regards, > Tung Dinh > > PhD student > The University of Georgia > Department of Chemistry > ------------------------------ > *From:* CCP4 bulletin board <[email protected]> on behalf of Tung > Thanh Dinh <[email protected]> > *Sent:* Friday, August 30, 2019 9:36:58 AM > *To:* [email protected] <[email protected]> > *Subject:* [ccp4bb] I am doing phenix refinement now. Is it a problem if > the r-work and r-free values are equal? > > > After phasing with phenix, i clicked on the phenix.refine ribbon. Since > then for every macrocycle of refinement, r-work and r-free values are > always the same. Is this a problem that I need to fix? > > > Kindest regards, > Tung Dinh > > PhD student > The University of Georgia > Department of Chemistry > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
