And if you log in to C6 (c6.csiro.au - one can use the guest account: click on "Or click here to try guest account") - navigate to "screens list"=>"commercial screens" => KERAfast => and if you click on the screen then it will come up with a description of what is in every well, and (most) of the conditions will also have recipes of how to make that condition.
Cheers, Janet Janet Newman Principal Scientist / Director, Collaborative Crystallisation Centre (C3) CSIRO Material Science and Engineering 343 Royal Parade Parkville. VIC. 3052 Australia Tel +613 9662 7326 Email [email protected] ________________________________ From: CCP4 bulletin board <[email protected]> on behalf of Guillaume Gaullier <[email protected]> Sent: Wednesday, October 16, 2019 1:45 AM To: [email protected] <[email protected]> Subject: Re: [ccp4bb] Crystal screen for DNA binding proteins Hi Sylvia, A screen specifically designed to crystallize protein-DNA complexes is described in this paper: https://www.ncbi.nlm.nih.gov/pubmed/22869139 Back when it was just published, I manually prepared the 48 conditions and it was not too difficult (each one is a combination of one buffer, one or two salts, and one PEG). Now you can buy it pre-made from at least one supplier (possibly available elsewhere too, I haven’t checked): https://www.kerafast.com/product/287/protein-nucleic-acid-complex-crystal-screen Good luck with your project! Guillaume On Oct 15, 2019, at 01:48, Sylvia Fanucchi <[email protected]<mailto:[email protected]>> wrote: Hi Can anyone recommend a good crystal screen to use to crystalise protein-NA complexes? Kind Regards Sylvia This communication is intended for the addressee only. It is confidential. If you have received this communication in error, please notify us immediately and destroy the original message. You may not copy or disseminate this communication without the permission of the University. Only authorised signatories are competent to enter into agreements on behalf of the University and recipients are thus advised that the content of this message may not be legally binding on the University and may contain the personal views and opinions of the author, which are not necessarily the views and opinions of The University of the Witwatersrand, Johannesburg. All agreements between the University and outsiders are subject to South African Law unless the University agrees in writing to the contrary. ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
