It happens. Ef-Tu is methylated on a single lysine
Also LafV is a putative methylase in E. Coli but its effects were not studied in detail. Salmonella methylates flagellin which is how lysine methylation was discovered - and E.coli is a kissing cousin so... Artem On Tue, Nov 5, 2019, 12:31 Jennifer Fleming <jennifer.flem...@uni.kn> wrote: > > Dear CCP4 community, > > > > I am hoping that someone here might know the answer to this as I cannot > find anything concrete in my literature search. > > > > I have recently received a mass spec result indicating that a recombinant > protein expressed in *E. coli* is heavily lysine methylated. I would like > to know is this an artefact and is it even possible for *E. coli* to > methylate lysines? > > > > Thank you for any help, > > > > Jen > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
