Dear Matthias,
do you have an idea of what triggered this low completeness at high
resolution?
Is it that the crystal still diffracts in one direction of space but
stops in another, resulting in low spherical completeness? If so, then
you can use all of your good data, and I would probably go further than
your 1.8A resolution.
Best regards
Vincent
Le 25/11/2019 à 14:11, Matthias Oebbeke a écrit :
Dear ccp4 Bulletin Board,
I collected a dataset at a synchrotron beamline and got the statistics
(CORRECT.LP) after processing (using xds) shown in the attached pdf-file.
Do you think this dataset is usable, due to its low completeness? As
you can see in the attached file the completeness is just 50% in the
highest resolution shell, whereas the I over Sigma is above 2 and also
the CC 1/2 (80%) and the R factors (36.8%) have reasonable values.
Furthermore the overall statistic are good regarding R factor, CC 1/2
and I over Sigma. The only problem seems to be the completeness. If I
would set the cut-off at a lower resolution to get good completeness,
I would throw away nearly half of my reflections.
I'm happy to here your opinion. In Addition to that: The space group
is orthorhombic and the dataset was collected over 120° using fine
slicing (0.1°).
Best regards,
Matthias Oebbeke
--
Vincent Chaptal, PhD
MMSB -UMR5086
Drug Resistance and Membrane Proteins Laboratory
7 passage du Vercors
69007 LYON
FRANCE
+33 4 37 65 29 01
http://mmsb.cnrs.fr/en/
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