Dear Armando, in case you have enough material to spare, I would use a concentrator to create a saturated solution, i.e. concentrate until it precipitates. Measure the concentration of the saturated solution, i.e., supernatant without disturbing the solution with the precipitate.
You can start crystallisation trials at 70-90% of this concentration. Best, Tim On Wednesday, January 8, 2020 5:16:31 PM CET Armando Albert wrote: > Dear all, > I was wondering how to guess the optimal protein concentration for the > initial crystallisation trials. Is there any trick or assay other than the > classic PCT from Hampton? Armando > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 -- -- Tim Gruene Head of the Centre for X-ray Structure Analysis Faculty of Chemistry University of Vienna Phone: +43-1-4277-70202 GPG Key ID = A46BEE1A ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
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