For general information, HEKs were derived from a legally aborted foetus the in the Netherlands in 1973. Not "killed children".
(As a side note, such use of emotive language is counter-productive and often the tool of those who seek remove reproductive rights and autonomy from women. I'm certain we wouldn't want to be tarred with that brush.) That said, use CHOs if you have an issue with HEKs. The only potential problem would be if what you wanted to study required authentic human post-translational modifications, and even then, I imagine CHOs would still be a suitable surrogate in the majority of cases. Cheers, Dave -- Dr David C. Briggs Senior Laboratory Research Scientist Signalling and Structural Biology Lab The Francis Crick Institute London, UK == about.me/david_briggs ________________________________ From: Petr Kolenko <petr.kole...@fjfi.cvut.cz> Sent: Saturday, January 25, 2020 7:10:06 AM To: David Briggs <david.bri...@crick.ac.uk>; CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: RE: [ccp4bb] protein expression in human cells Dear colleagues, I wonder if there were a bit less controversial possibility. No matter if that was less efficient. Would there be an option of using human cell lines that do not origin from killed children? As far as I know, the HEK cells do. Sometimes, the science can be pretty cruel. I am sorry for opening of this topic on a crystallographic forum, but so far, nobody has convinced me that I am allowed to work with these cell lines from a humanity (and Christian) point of view. Best regards, Petr From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of David Briggs Sent: Saturday, January 25, 2020 7:49 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] protein expression in human cells Hi Gloria, Another vote here for HEK 293 Expi or Freestyle. The off-the-shelf transfection reagents are super expensive, but I make my own (happy to share protocols with anyone interested) and we normally get a few mgs of protein per litre of suspension culture -- certainly enough for crystallography. If budget is an issue, semi-stable transfection of adherent HEK293s is much cheaper but the turnaround from vector to protein is much slower (a few weeks rather than a few days), and growing up panels of mutants can be quite tedious. Cheers & good luck Dave -- Dr David C. Briggs Senior Laboratory Research Scientist Signalling and Structural Biology Lab The Francis Crick Institute London, UK == about.me/david_briggs ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>> on behalf of Rezaul Karim <00001e364c8f16de-dmarc-requ...@jiscmail.ac.uk<mailto:00001e364c8f16de-dmarc-requ...@jiscmail.ac.uk>> Sent: Saturday, January 25, 2020 1:31:02 AM To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> <CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: Re: [ccp4bb] protein expression in human cells In our lab, we see Expi293F works very good. Thanks, Reza Md. Rezaul Karim Graduate student, PhD Program in Integrated Biomedical Sciences Morsani College of Medicine University of South Florida E-mail: reza...@yahoo.com<mailto:reza...@yahoo.com>, rez...@health.usf.edu<mailto:rez...@health.usf.edu> Phone: +1-954-937-8487 On Friday, January 24, 2020, 4:51:11 PM EST, Gloria Borgstahl <gborgst...@gmail.com<mailto:gborgst...@gmail.com>> wrote: Hello CCP4-ers, I was wondering what people have found to be the best human cell line expression system for making a large quantity of purified recombinant protein. Any information and protocols would be greatly appreciated. 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