HI Ben

In mmcif we use _exptl_crystal_grow.pdbx_details.
See
http://mmcif.wwpdb.org/dictionaries/mmcif_pdbx_v50.dic/Items/_exptl_crystal_grow.pdbx_details.html


John


On 26 Jan 2020, 18:02 +0000, benjamin bax <[email protected]>, wrote:
>
> In PDB files you could put all information about protein delivery buffer, 
> crystallisation conditions and cryo conditions in
> REMARK 280
>
> (and pull it through in refmac with keyword
> pdbout copy remarks 280
> )
>
> Where are you meant to put all the information about what could be in your 
> crystal in mmcif_pdbx?
>
> Thanks, Ben
>
> Web (below) does not seem to give any very clear answers and I could not work 
> it out from looking at half a dozen recent pdb entries.
> Data item below is used in zero depositions. ..
>
>
> Data items in the PDBX_EXPTL_CRYSTAL_GROW_COMP category record
>                details about the components of the solutions that were 'mixed'
>                to produce the crystal.
>              
> http://mmcif.wwpdb.org/dictionaries/mmcif_pdbx_v40.dic/Categories/pdbx_exptl_crystal_grow_comp.html
>
> loop_
> _pdbx_exptl_crystal_grow_comp.crystal_id
> _pdbx_exptl_crystal_grow_comp.sol_id
> _pdbx_exptl_crystal_grow_comp.comp_id
> _pdbx_exptl_crystal_grow_comp.comp_name
> _pdbx_exptl_crystal_grow_comp.conc
> _pdbx_exptl_crystal_grow_comp.conc_range
> _pdbx_exptl_crystal_grow_comp.conc_units
> 4    'protein'     1  'protein'   25.    .  'mg/ml'
> 4    'protein'     2  'Tris HCl'  20.    .  'millimolar'
> 4    'protein'     3  'NaCl'       0.2   .  'molar'
> 4    'precipitant' 1  'PEG 4000'  12.5   .  'percent_weight_by_volume'
> 4    'precipitant' 2  'MES'        0.1   .  'molar'
> pdbout copy remarks 280
>
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