Hi Apropos Mosflm - if you have HDF5 files from ESRF (or Diamond, probably elsewhere) you can sum the images internally to whatever rotation range per pseudo image you want (so if you have, say, 0.05º physical images you could process 0.1, 0.15, 0.20º, etc), provided you have installed Mosflm 7.3.* (not yet distributed as part of CCP4, as far as I can work out [apologies to CCP4 core group if they’ve done this and I haven’t noticed], although it’s been available for quite some time…).
I wouldn’t recommend summing to 1.0º unless the mosaicity is really quite large - iMosflm itself will suggest an appropriate value, and in my limited experience of processing reasonable quality datasets (during testing) the optimum often turns out to be ~0.2 - 0.5º (based on metrics like CC-1/2, Rmeas, I/sig(I) etc). Just my two ha’porth Harry > On 12 Feb 2020, at 07:57, Wim Burmeister <wim.burmeis...@ibs.fr> wrote: > > Hello, > > do you have some details about the space group ? Did the integration not miss > any sports ? I would rather think of an ncs close to crystallographic > symmetry, or maybe some twinning problem. > I guess these are Pilatus data, can you combine the frames into 1 degree > oscillations and try Mosflm processing to see how the patterns integrate ? > > Greetings > > Wim > Le 11/02/2020 à 22:31, Daniele de Sanctis a écrit : >> Hi all, >> >> I'm currently dealing with what I think it is a case of LTD (off-origin >> Patterson peak, with vector along w of ~ 7A and electron density map showing >> a "ghost" map shifted by 7 A). I saw there are quite a few cases reported in >> literature (for example Hare et al, 2006), but what I could not find is how >> I can demodulate the data. Is there any software that can be used for this? >> >> Thank you >> >> Daniele >> >> -- >> ἀρετή >> --------------------------------------- >> Daniele de Sanctis, PhD >> >> Structural Biology Group >> ESRF, Grenoble, France >> Tel 33 (0)4 76 88 2869 >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1>-- > Wim Burmeister > Professeur > Institut de Biologie Structurale (IBS) CIBB > 71 avenue des Martyrs / CS 20192 > 38044 Grenoble Cedex 9, FRANCE > E-mail: wim.burmeis...@ibs.fr <mailto:wim.burmeis...@ibs.fr> > Tel: +33 (0) 457 42 87 41 Fax: +33 (0) 476 20 94 00 > website > <http://www.ibs.fr/research/research-groups/viral-replication-machines-group-m-jamin/team-03/article/poxvirus-replication-machinery-presentation/> > map > <https://www.openstreetmap.org/?mlat=45.20762&mlon=5.69255#map=17/45.20762/5.69255> > > Changement climatique : «Les autres combats n’ont aucun sens si celui-là est > perdu» (Aurélien Barrau) > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
