Hi, you mentioned that there is no electron density for your 5th domain. If there is nothing coming up even after refinement of the four domains, than maybe your 5th domain is not there, or so disordered that it doesn't show up.
Christian On Wed, Feb 12, 2020 at 9:47 AM RAJNESH KUMARI YADAV <[email protected]> wrote: > Thanks Clemens and Lumbini for your help. > I had tried both the things but none of them worked for this problem. > > On Wed, Feb 12, 2020 at 1:56 PM Clemens Vonrhein < > [email protected]> wrote: > >> Hi, >> >> MOLREP [1] has a nice feature of searching for structures in electron >> density - with the known parts of your model fixed. Basically follow >> the recipe in [1] (search for "refmac.mtz" - but you can use any other >> set of amplitudes/phases as well). In your case the 4-domain model >> would be given to the -mx flag and the 5th domain search model to -m. >> >> This worked for me a few times in the past ... >> >> Cheers >> >> Clemens >> >> [1] http://www.ccp4.ac.uk/html/molrep.html >> >> On Wed, Feb 12, 2020 at 07:12:48AM +0000, Rajnesh Kumari Yadav wrote: >> > Hello everyone, >> > >> > I am working on a protein which have 5 domain in it, we have its 4 >> domain structure and 3.0 Angstrom data of 5 domain protein crystal. While >> doing the Molecular replacement we got solution with four domain with good >> electron density and space for last domain without any density. When we >> tried Molecular replacement with the missing domain (modelled) only we got >> solution shows density for this missing domain but not able see electron >> density for rest four domain even though room for 4 domain was visible in >> the solution. I need help or suggestion for this issue. >> > >> > ######################################################################## >> > >> > To unsubscribe from the CCP4BB list, click the following link: >> > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> >> -- >> >> *-------------------------------------------------------------- >> * Clemens Vonrhein, Ph.D. vonrhein AT GlobalPhasing DOT com >> * Global Phasing Ltd., Sheraton House, Castle Park >> * Cambridge CB3 0AX, UK www.globalphasing.com >> *-------------------------------------------------------------- >> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
