Hi All, How is the 'correct' resolution estimation related to the estimated error on some observed hydrogen bond length of interest, or an error on the estimated occupancy of a ligand or conformation or anything else that has structural significance?
In crystallography, it isn't really (only in some very approximate fashion), and I doubt that in EM there is something to that effect. If you want to use the resolution to get a gut feeling on how your maps look and how your data behaves, it doesn't really matter what standard you use, as long as you are consistent in the use of the metric you use. If you want to use this estimate to get to uncertainties of model parameters, you better try something else. Regards Peter Zwart On Sun, Feb 16, 2020 at 8:38 AM Marin van Heel < [email protected]> wrote: > Dear Pawel and All others .... > > This 2010 review is - unfortunately - largely based on the flawed > statistics I mentioned before, namely on the a priori assumption that the > inner product of a signal vector and a noise vector are ZERO (an > orthogonality assumption). The (Frank & Al-Ali 1975) paper we have refuted > on a number of occasions (for example in 2005, and most recently in our > BioRxiv paper) but you still take that as the correct relation between SNR > and FRC (and you never cite the criticism...). > Sorry > Marin > > On Thu, Feb 13, 2020 at 10:42 AM Penczek, Pawel A < > [email protected]> wrote: > >> Dear Teige, >> >> I am wondering whether you are familiar with >> >> Resolution measures in molecular electron microscopy. >> Penczek PA. Methods Enzymol. 2010. >> Citation >> >> Methods Enzymol. 2010;482:73-100. doi: 10.1016/S0076-6879(10)82003-8. >> >> You will find there answers to all questions you asked and much more. >> >> Regards, >> Pawel Penczek >> >> >> Regards, >> Pawel >> _______________________________________________ >> 3dem mailing list >> [email protected] >> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem >> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- ------------------------------------------------------------------------ P.H. Zwart Staff Scientist Molecular Biophysics and Integrated Bioimaging & Center for Advanced Mathematics for Energy Research Applications Lawrence Berkeley National Laboratories 1 Cyclotron Road, Berkeley, CA-94703, USA Cell: 510 289 9246 PHENIX: http://www.phenix-online.org CAMERA: http://camera.lbl.gov/ ------------------------------------------------------------------------- ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
