*Membrane Protein Expression, Purification and Characterization (mPEPC2)*
EMBO Practical Course, 7-14 September 2020, Hamburg, Germany
Organisers: Maria Garcia Alai, Eric Geertsma, Christian Löw and Kim Remans
https://meetings.embo.org/event/20-mpepc2
Integral membrane proteins (IMPs) are encoded by 20-30% of all open
reading frames and provide key functions in the cell. Due to their
hydrophobic nature, they are more difficult to study and structural
information is lagging far behind their soluble counterparts. This EMBO
Practical Course trains PhD students and early career postdocs with the
latest developments in membrane protein production, characterization and
structure determination methods. Modern protein expression techniques in
various hosts will be presented and students will get acquainted with
state-of-the-art approaches for studying structural and dynamic aspects
of membrane proteins. An attitude to combine protein purification and
characterization will be taught to impress the necessity on the students
that proteins have to be conditioned with care to achieve structural
information. The presence of a crystallization and cryo-EM facility as
well as beamlines for SAXS and macromolecular crystallography at the
venue will be employed to perform integrated structural biology
experiments. The course has an emphasis on practicals in small groups,
and a "Meet the scientist" session at the beginning of the course will
ensure a close interaction between students and tutors. The aim is to
inspire the students to apply the newly acquired knowledge and to
maintain interactions among themselves and the tutors afterwards.
Accepted students are encouraged to bring their own samples (frozen
state) for quality control, crystallization and negative stain EM.
Precise shipment conditions and information will be provided to the
selected applicants.
*Application deadline: 15 June 2020*
Looking forward to receiving your applications!
Sarah
on behalf of the course organisers
Sarah Marshall-Bensch
European Molecular Biology Laboratory, Hamburg Unit
E:[email protected] <mailto:[email protected]>
T: +49 40 89902 111
F: +49 40 89902 260
EMBL c/o DESY Notkestraße 85 22607 Hamburg, Germany
www.embl-hamburg.de <http://www.embl-hamburg.de>
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