Hi Chitra Latka, By far the best approach is to find what protein is interacting with the one you have the structure and try co-crystallizing them together. At least there will be some more biology (science) involved in what you will be doing. You may get lucky and get different packing of your full length protein and get some sort of structure for last 20 aa. Then what?
Regards, From: CCP4 bulletin board <[email protected]> On Behalf Of chitra latka Sent: Thursday, March 12, 2020 3:54 AM To: [email protected] Subject: [ccp4bb] Flexible C terminus Dear All, I am working on a protein that has flexible C terminus. None of the available structures even in homologs have density for C term region (around 20 odd residues). All the available pdb entries have missing density for these 20 residues at C terminus. I am going to try my luck crystallising the entire protein in hope of getting density for C term residues as well (Fingers crossed). Has anyone faced a similar problem where they have managed to get density for a flexible terminus successfully? Any suggestions would be appreciated. Cheers ! Chitra Latka ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://clicktime.symantec.com/3K2tGPDPQHVzmmXDiGLP6PM6H2?u=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
