Hello, I haven't seen this before but doing a round of refinment with REFMAC, after molecular replacement with phaser, my R factor and R free have increased?
Also is it weird that my Rfree is smaller than my R factor? Result: Initial Final R factor 0.3621 0.3761 R free 0.3108 0.4733 Rms BondLength 0.0076 0.0057 Rms BondAngle 1.6504 1.5193 Rms ChirVolume 0.0600 0.0526 My map was ok(ish) but there was indication of anistropy so I attempted to improve it by using STARANISO and re-ran phaser with the output, and this is the results I see following REFMAC. Thanks, Kyle ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
